Infecting Larval Arthropods with a Chimeric, Double Subgenomic Sindbis Virus Vector to Express Genes of Interest
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چکیده
منابع مشابه
A Bacteriophage-Related Chimeric Marine Virus Infecting Abalone
Marine viruses shape microbial communities with the most genetic diversity in the sea by multiple genetic exchanges and infect multiple marine organisms. Here we provide proof from experimental infection that abalone shriveling syndrome-associated virus (AbSV) can cause abalone shriveling syndrome. This malady produces histological necrosis and abnormally modified macromolecules (hemocyanin and...
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15 صفحه اولSpecific nucleotide changes in the subgenomic promoter region influence infectivity of the sindbis virus.
Transcription of the subgenomic mRNA of Sindbis virus (SINV) is initiated at a subgenomic promoter (SP). Alignment of SINV sequences identified a 68-nucleotide conserved domain spanning -19 to +49 relative to the subgenomic mRNA start site. Nucleotide T or C is present at -18 or +49 in all known SINVs while a Sindbis-like virus XJ-160 has an A or T at a corresponding position. Our results indic...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99275bm06